Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Figure 1: Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using HSP60 mouse mAb with DAB staining.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 mouse mAb with DAB staining.Application
Figure 4: Immunofluorescence analysis of 3T3-L1 cells using HSP60 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 5: Flow cytometric analysis of Hela cells using HSP60 mouse mAb (green) and negative control (purple).| Product Name | HSP60 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; HSPD1 |
| Immunogen | Purified recombinant fragment of human HSP60 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRatMouseMonkey |
| Alternative Names | HLD4 CPN60 GROEL HSP60 HSP65 SPG13 HSP-60 HuCHA60 HSPD1 |
|---|---|
| Molecular Weight(MW) | 61kDa |
| Entrez Gene | 3329 |
|---|
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Figure 1: Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using HSP60 mouse mAb with DAB staining.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 mouse mAb with DAB staining.
Application
Figure 4: Immunofluorescence analysis of 3T3-L1 cells using HSP60 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 5: Flow cytometric analysis of Hela cells using HSP60 mouse mAb (green) and negative control (purple).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
