Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.Application
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Application
Figure 3: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).| Product Name | ACTA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AAT6; ACTSA; ACTA2 |
| Immunogen | Purified recombinant fragment of human ACTA2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBICCFCMICC/IF |
| WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkeyRatMouse |
| Alternative Names | AAT6 ACTSA ACTA2 |
|---|---|
| Molecular Weight(MW) | 42kDa |
| Entrez Gene | 59 |
|---|
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.
Application
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 3: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
