Application
Figure 1: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.Application
Figure 3: Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 4: Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).| Product Name | SMAD5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5 |
| Immunogen | Purified recombinant fragment of human SMAD5 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Alternative Names | Dwfc JV5-1 MADH5 DKFZp781C1895 DKFZp781O1323 SMAD5 |
|---|---|
| Molecular Weight(MW) | 52kDa |
| Entrez Gene | 4090 |
|---|
Application
Figure 1: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
Application
Figure 3: Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 4: Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
