Application
Figure 1: Western blot analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.Application
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 mouse mAb with DAB staining.Application
Figure 4: Flow cytometric analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).| Product Name | SMAD4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JIP; DPC4; MADH4; SMAD4 |
| Immunogen | Purified recombinant fragment of human SMAD4 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | JIP DPC4 MADH4 SMAD4 |
|---|---|
| Molecular Weight(MW) | 65kDa |
| Entrez Gene | 4089 |
|---|
Application
Figure 1: Western blot analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.
Application
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 mouse mAb with DAB staining.
Application
Figure 4: Flow cytometric analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
