Application
Figure 1: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.Application
Figure 3: Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.Application
Figure 4: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).| Product Name | DDX4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VASA;MGC111074;DDX4 |
| Immunogen | Purified recombinant fragment of human DDX4 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | VASA MGC111074 DDX4 |
|---|---|
| Molecular Weight(MW) | 76kDa |
| Entrez Gene | 54514 |
|---|
Application
Figure 1: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
Application
Figure 3: Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Application
Figure 4: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
