Application
Figure 1: Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).Application
Figure 2: Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.Application
Figure 4: Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 5: Confocal immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 6: Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 7: Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).| Product Name | CD44 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL |
| Immunogen | Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCFCMICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Alternative Names | IN LHR MC56 MDU2 MDU3 MIC4 Pgp1 CDW44 CSPG8 HCELL |
|---|---|
| Molecular Weight(MW) | 82kDa |
| Entrez Gene | 960 |
|---|
Application
Figure 1: Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Application
Figure 2: Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.
Application
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.
Application
Figure 4: Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 5: Confocal immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 6: Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 7: Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
