Application
Figure 1: Western blot analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.Application
Figure 3: Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Product Name | Nucleophosmin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B23; NPM |
| Immunogen | Purified recombinant fragment of human NPM (2-265) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkey |
| Alternative Names | B23 NPM |
|---|---|
| Molecular Weight(MW) | 33kDa |
| Entrez Gene | 4869 |
|---|
Application
Figure 1: Western blot analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.
Application
Figure 3: Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
