Application
Figure 1: Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.Application
Figure 3: Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | MLH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FCC2; COCA2; HNPCC |
| Immunogen | Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMonkey |
| Alternative Names | FCC2 COCA2 HNPCC |
|---|---|
| Molecular Weight(MW) | 85kDa |
| Entrez Gene | 4292 |
|---|
Application
Figure 1: Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.
Application
Figure 3: Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
