Application
Figure 1: Western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A mouse mAb with DAB staining.Application
Figure 3: Confocal immunofluorescence analysis of PC-12 cells using WNT5A mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | WNT5A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hWNT5A |
| Immunogen | Purified recombinant fragment of WNT5A expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Unknow |
| Tested Applications | WBIHCICCICC/IF |
| WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Alternative Names | hWNT5A |
|---|---|
| Molecular Weight(MW) | 42.3kDa |
| Entrez Gene | 7474 |
|---|
Application
Figure 1: Western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A mouse mAb with DAB staining.
Application
Figure 3: Confocal immunofluorescence analysis of PC-12 cells using WNT5A mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Ascitic fluid containing 0.03% sodium azide. |
|---|---|
| Storage Instructions | 4℃; -20℃ for long term storage |
