Western blot
Fig1: Western blot analysis of MAdCAM-1 expression in ECV304 whole cell lysate.Western blot
Fig2: Western blot analysis of MAdCAM-1 expression in non-transfected (A) and mouse MAdCAM-1 transfected (B) 293T whole cell lysates.| Product Name | MAdCAM-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Addressin mucosal antibody hMAdCAM 1 antibody hMAdCAM-1 antibody MACAM1 antibody MADCA_HUMAN antibody MAdCAM 1 antibody MAdCAM-1 antibody Madcam1 antibody Mucosal addressin cell adhesion molecule 1 antibody |
| Product description | The recirculation of lymphocytes through different organs is thought to be regulated by adhesion molecules (“homing receptors”) recognizing tissue-specific vascular addressins on the endothelium. The mucosal vascular addressin, MadCAM-1 (mucosal addressin cell adhesion molecule 1), is an immunoglobulin superfamily adhesion molecule for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into Peyer’s patches and the intestinal lamina propria. MadCAM-1 acts as an endothelial cell ligand for leukocyte homing receptors L-Selectin and Integrin α4/β7. MadCAM-1 is strongly expressed on inflamed portal vein/sinusoidal endothelium in autoimmune-mediated liver disease and plays a major contributory role in the progression of chronic experimental autoimmune encephalomyelitis. |
| Immunogen | Endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Rat |
| Tested Applications | FCIFIPWB |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 FC:1 μg per 1 x 106 cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | Addressin mucosal antibody hMAdCAM 1 antibody hMAdCAM-1 antibody MACAM1 antibody MADCA_HUMAN antibody MAdCAM 1 antibody MAdCAM-1 antibody Madcam1 antibody Mucosal addressin cell adhesion molecule 1 antibody |
|---|---|
| Molecular Weight(MW) | 40/29 kDa |
| Cellular Localization | Membrane |
| SwissProt ID | Q13477 |
|---|
Western blot
Fig1: Western blot analysis of MAdCAM-1 expression in ECV304 whole cell lysate.
Western blot
Fig2: Western blot analysis of MAdCAM-1 expression in non-transfected (A) and mouse MAdCAM-1 transfected (B) 293T whole cell lysates.| Positive Control | ECV304, 293T. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 FC:1 μg per 1 x 106 cells |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
