ICC/IF
Immunofluorescence analysis of T24 cells using PI3K p85 alpha antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.ICC/IF
Immunofluorescence analysis of HepG2 cells using PI3K p85 alpha antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-PI3K p85 alpha + Gamma antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFFC |
| IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3R1 |
|---|---|
| Gene Synonyms | AGM7 GRB1 IMD36 p50alpha p55alpha p85 p85-ALPHA p85alpha PI3K PI3KA |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 1 |
| Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Alternative Names | growth factor receptor bound 1 p55-GAMMA p55PIK p85-alpha GRB1 phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha phosphatidylinositol 3-kinase p85 alpha phosphatidylinositol 3-kinase regulatory subunit gamma phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphatidylinositol 3-kinase-associated p-85 alpha Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50) Phosphoinositide 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphoinositide-3-kinase regulatory subunit alpha phosphoinositide-3-kinase regulatory subunit 1 (alpha) phosphoinositide-3-kinase regulatory subunit 1 (p85 alpha) phosphoinositide-3-kinase regulatory subunit polypeptide 1 (p85 alpha) PI3 kinase-associated p85 PI3-kinase p85 subunit alpha PI3-kinase p85-subunit alpha PI3-kinase regulatory subunit alpha PI3-kinase regulatory subunit gamma PI3-kinase subunit p55-gamma PI3-kinase subunit p85-alpha PI3K regulatory subunit alpha PI3K regulatory subunit gamma PI3K-p46 polypeptide 1 p85 alpha ptdIns-3-kinase p85-alpha ptdIns-3-kinase regulatory subunit alpha PtdIns-3-kinase regulatory subunit p85-al ptdIns-3-kinase regulatory subunit p85-alpha RP4-533D7.2 |
| Molecular Weight(MW) | 83 kDa（Observed:56 kDa） |
| Source | Rabbit |
| Cellular Localization | Cytoplasmic |
| SwissProt ID | P27986 |
|---|
ICC/IF
Immunofluorescence analysis of T24 cells using PI3K p85 alpha antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.
ICC/IF
Immunofluorescence analysis of HepG2 cells using PI3K p85 alpha antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Application Notes | IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
