IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using Nrf2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using Nrf2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.WB
(B–D) Application of Western blot (B, C) and qT-PCR (D) to ascertain the protein and mRNA expression levels, respectively, in maternal placenta, liver, and pancreas tissues.WB
(B, C) Western blotting analysis was used to detect theNrf2 and CBR1 expression level at week 0 and 16 in offspring rat liver (B) and pancreas (C).WB
(A) BRL cells were exposed to 25-hydroxyvitaminD (VD3) at 0, 10, 100, 1,000 nM for 24 h, and thenNrf2 levels were evaluated by Western blot. (B) BRL cells were exposed to VD3 (1 μM) with or without Brusatol (160 nM) or siCBR1. Next, the level of Nrf2 was determined by Western blotting.WB
(b–d) Western blot (b,c) and qRT-PCR (d) to ascertain the protein and mRNA expression levels in maternal placenta tissues.WB
Western blot analysis using Nrf2 antibody against Mouse testes(1), Mouse stomach(2),Rat stomach(3),Rat serum(4) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-Nrf2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| WB:1:1000-1:2000 IHC:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NFE2L2 |
|---|---|
| Gene Synonyms | IMDDHH Nrf-2 NRF2 |
| Gene Full Name | nuclear factor, erythroid 2 like 2 |
| Gene Summary | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | E4TF1-60 E4TF1A NF-E2-like basic leucine zipper transcriptional activator NF-E2-related factor 2 NFE2-related factor 2 NFT2 NRF2 NRF2A nuclear factor erythroid-derived 2-like 2 nuclear factor erythroid 2 like 2 nuclear factor erythroid 2-like 2 nuclear factor erythroid derived 2 like 2 transcription factor |
| Molecular Weight(MW) | 68kDa |
| Source | Rabbit |
| Cellular Localization | Cytosol;Nucleus |
| SwissProt ID | Q16236 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using Nrf2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using Nrf2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
WB
(B–D) Application of Western blot (B, C) and qT-PCR (D) to ascertain the protein and mRNA expression levels, respectively, in maternal placenta, liver, and pancreas tissues.
WB
(B, C) Western blotting analysis was used to detect theNrf2 and CBR1 expression level at week 0 and 16 in offspring rat liver (B) and pancreas (C).
WB
(A) BRL cells were exposed to 25-hydroxyvitaminD (VD3) at 0, 10, 100, 1,000 nM for 24 h, and thenNrf2 levels were evaluated by Western blot. (B) BRL cells were exposed to VD3 (1 μM) with or without Brusatol (160 nM) or siCBR1. Next, the level of Nrf2 was determined by Western blotting.
WB
(b–d) Western blot (b,c) and qRT-PCR (d) to ascertain the protein and mRNA expression levels in maternal placenta tissues.
WB
Western blot analysis using Nrf2 antibody against Mouse testes(1), Mouse stomach(2),Rat stomach(3),Rat serum(4) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 IHC:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
