WB
Western blot analysis using Cyclin D1 antibody against Hep G2 (1), T24 (2), Hela (3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using Cyclin D1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using Cyclin D1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Cyclin D1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using Cyclin D1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using Cyclin D1 antibody with DAB staining.| Product Name | Anti-Cyclin D1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CCND1 |
|---|---|
| Gene Synonyms | BCL1 PRAD1 U21B31 D11S287E |
| Gene Full Name | cyclin D1 |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers. [provided by RefSeq, Dec 2019] |
| Molecular Weight(MW) | 34 kDa（Observed: 40 kDa） |
| Cellular Localization | Nucleus |
| SwissProt ID | P24385 |
|---|
WB
Western blot analysis using Cyclin D1 antibody against Hep G2 (1), T24 (2), Hela (3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using Cyclin D1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using Cyclin D1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Cyclin D1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using Cyclin D1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using Cyclin D1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
