WB
Western blot analysis using NCOR1 antibody against PANC-1 (1), CACO2 (2) cell lysate.10% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using NCOR1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using NCOR1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using NCOR1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using NCOR1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using NCOR1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using NCOR1 antibody with DAB staining.| Product Name | Anti-NCOR1 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NCOR1 |
|---|---|
| Gene Synonyms | N-CoR TRAC1 N-CoR1 hN-CoR PPP1R109 |
| Gene Full Name | nuclear receptor corepressor 1 |
| Gene Summary | This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010] |
| Molecular Weight(MW) | 270 KDa |
| Source | Rabbit |
| Cellular Localization | Nucleus |
| SwissProt ID | O75376 |
|---|
WB
Western blot analysis using NCOR1 antibody against PANC-1 (1), CACO2 (2) cell lysate.10% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using NCOR1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using NCOR1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using NCOR1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using NCOR1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using NCOR1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using NCOR1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
