| Product Name | TBST (10X) (TBS with Tween-20, 10X) |
|---|---|
| Product Description | TBST (10X) (TBS with Tween - 20) can be used for preparing blocking solutions, preparing primary or secondary antibodies, and washing after primary or secondary antibody incubations in experiments such as WB (Western blot), Immunofluorescence (IF), IHC (Immunohistochemistry), and ICC (Immunocytochemistry). Blocking and washing can reduce background and enhance the signal - to - noise ratio. This product is a 10X concentrated solution and must be dissolved with distilled water or deionized water to prepare a 1X solution before use. For example, to prepare 1 L of TBST (1X), take 100 ml of TBST (10X) and add 900 ml of deionized water and mix well. The TBST (1X) solution contains 20 mM Tris, 137 mM NaCl, 0.1% Tween - 20, with a pH of 7.6 ± 0.1 (25°C). For washing membranes in Western blot experiments, calculate based on 5 - 10 ml of TBST (1X) required for each membrane wash. Each 100 ml of TBST (10X) can be used for 100 - 200 washes. For immunostaining and other experiments, calculate based on 0.2 - 1 ml of TBST (1X) required for each sample wash. Each 100 ml of TBST (10X) can be used to wash 1000 - 5000 samples. |
| Storage Condition | Store at 4°C, valid for one year. It can be stored at -20°C for long - term preservation. When stored at room temperature, it is valid within one month. |
| Precautions |
Data sheet for OM750016