WB
Western blot analysis using LIMK1 antibody against NIH3T3(1), MCF-7(2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using LIMK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using LIMK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using LIMK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using LIMK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using LIMK1 antibody with DAB staining.| Product Name | Anti-LIMK1 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LIMK1 |
|---|---|
| Gene Synonyms | LIMK LIMK-1 |
| Gene Full Name | LIM domain kinase 1 |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cell projection, Cytoplasm, Cytoskeleton, Nucleus |
| SwissProt ID | P53667 |
|---|
WB
Western blot analysis using LIMK1 antibody against NIH3T3(1), MCF-7(2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using LIMK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using LIMK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using LIMK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using LIMK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using LIMK1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
