WB
Western blot analysis using RPA2 antibody against Hek293(1) cell lysate.WB
Western blot analysis using RPA2 antibody against Rat intestine tissue(1),Rat stomach tissue(2),Mouse muscle tissue(3),Rat liver tissue(4) .| Product Name | Anti-RPA2 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPA2 |
|---|---|
| Gene Synonyms | RFA2 REPA2 RPA32 RP-A p32 RP-A p34 |
| Gene Full Name | replication protein A2 |
| Gene Summary | This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017] |
| Molecular Weight(MW) | 29KD,38KD |
| Cellular Localization | Nucleus |
| SwissProt ID | P15927 |
|---|
WB
Western blot analysis using RPA2 antibody against Hek293(1) cell lysate.
WB
Western blot analysis using RPA2 antibody against Rat intestine tissue(1),Rat stomach tissue(2),Mouse muscle tissue(3),Rat liver tissue(4) .| Application Notes | WB:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
