| Product Name | Matrigengel Matrix |
|---|---|
| Product description | Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound healing. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells. Matrigengel Matrix is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.Mogengel Matrigengel Matrixat 37 °C to form a reconstituted basement membrane. The major components of Mogengel Matrigengel Matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycan. |
| Source | Product Parameter Source: Mouse Tumor Appearance： ① Color: phenol-containing red matrigengel is yellow-pink, and phenol-free red matrix gum is translucent light yellow; ② Form: standard matrigengel dissolved at 4℃, a transparent liquid state; H |
| Storage Instructions | Thick Gel Method: 1. Thaw Matrigengel as stated above. 2. Mix Matrigengel by slowly pipetting solution up and down; be careful not to introduce air bubbles. 3. Pipette 200-300 μL per cm2 onto the growth surface. 4. Place coated object at 37 °C for 30 minutes. 5. Coated objects are ready for use. Thin Layer Method (non-gelling): 1. Thaw Matrigengel as stated above. 2. Mix Matrigengel by slowly pipetting solution up and down; be careful not to introduce air bubbles. 3. Dilute Matrigengel to desired concentration in cold serum-free medium. A 1:100 dilution is recommended for the propagation of primary cells. Empirical determination of the optimal coating concentration for your application may be required. 4. Add a sufficient amount of solution to cover the entire growth surface area. A volume of 300 µL per cm2 is recommended. 5. Incubate coated object at room temperature for one hour. 6. Aspirate coating solution and immediately plate cells. Do not allow coated surface to dry. |
Data sheet for OM642713