IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer using METTL3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.ICC/IF
Confocal immunofluorescence analysis of Hela cells using METTL3 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.| Product Name | [KD Validated] METTL3 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | METTL3 |
|---|---|
| Gene Synonyms | M6A IME4 Spo8 MT-A70 hMETTL3 |
| Gene Full Name | methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit |
| Gene Summary | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 64kDa(Observed MW 70kDa/75KDa) |
| Cellular Localization | Nucleus speckle. |
IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer using METTL3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ICC/IF
Confocal immunofluorescence analysis of Hela cells using METTL3 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
Data sheet for OM642761