WB
Western blot analysis of lysates from 1)mouse-brain, 2)Hela cells treated by UV 15min,3) HepG2 cells, primary antibody was diluted at 1:1000, 4°over night, secondary antibody HRP goat anti rabbit was diluted at 1:10000IHC
Immunohistochemical analysis of paraffin-embedded human Moderately differentiated hepatocellular carcinoma Antibody was diluted at 1:200(4° overnight).ICC/IF
Immunofluorescence analysis of un-treated (left) A549 and UV treated(right) A549 cell. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.| Product Name | GSDMD N-terminal rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from human GSDMD. AA range:1-100 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSDMD |
|---|---|
| Gene Synonyms | DF5L DFNA5L FKSG10 GSDMDC1 |
| Gene Full Name | gasdermin D |
| Gene Summary | Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009] |
| Target | The antibody detects endogenous full lenth and n-ternal fragment of gsdmd protein |
| Molecular Weight(MW) | GSDMD N-terminal:36kDa,GSDMD total:53kDa |
| Cellular Localization | [Gasdermin-D]: Cytoplasm, cytosol . Inflammasome . In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. .; [Gasdermin-D, N-terminal]: Cell membrane ; Multi-pass membrane protein . Secreted . Released in the extracellular milieu following pyroptosis. .; [Gasdermin-D, C-terminal]: Cytoplasm, cytosol . |
WB
Western blot analysis of lysates from 1)mouse-brain, 2)Hela cells treated by UV 15min,3) HepG2 cells, primary antibody was diluted at 1:1000, 4°over night, secondary antibody HRP goat anti rabbit was diluted at 1:10000
IHC
Immunohistochemical analysis of paraffin-embedded human Moderately differentiated hepatocellular carcinoma Antibody was diluted at 1:200(4° overnight).
ICC/IF
Immunofluorescence analysis of un-treated (left) A549 and UV treated(right) A549 cell. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
