WB
Western blot analysis using eIF2 alpha antibody against MCF-7(1) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using eIF2 alpha antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using eIF2 alpha antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using eIF2 alpha antibody with DAB staining.WB
Western blot analysis using eIF2 alpha antibody against Huvec(1),Panc-1(2), Cos-7(3),Nih3t3(4),Mcf-7(5) cell lysate.| Product Name | Anti-eIF2 alpha antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF2S1 |
|---|---|
| Gene Synonyms | EIF2 EIF-2 EIF2A EIF-2A EIF-2alpha |
| Gene Full Name | eukaryotic translation initiation factor 2 subunit alpha |
| Gene Summary | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| Molecular Weight(MW) | 36kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P05198 |
|---|
WB
Western blot analysis using eIF2 alpha antibody against MCF-7(1) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using eIF2 alpha antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using eIF2 alpha antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using eIF2 alpha antibody with DAB staining.
WB
Western blot analysis using eIF2 alpha antibody against Huvec(1),Panc-1(2), Cos-7(3),Nih3t3(4),Mcf-7(5) cell lysate.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
