WB
Western blot analysis of GABA B Receptor 2 on different lysates with Rabbit anti-GABA B Receptor 2 antibody at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.IF-P
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 2 with Rabbit anti-GABA B Receptor 2 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody( green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).FC
Flow cytometric analysis of SH-SY-5Y cells with GABA B Receptor 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | GABA B Receptor 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within C-terminal Human GABA B Receptor 2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:1000 IHC:1:50-1:200 IF-P:1:50 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GABBR2 |
|---|---|
| Gene Synonyms | HG20 DEE59 GPR51 EIEE59 GPRC3B NDPLHS GABABR2 HRIHFB2099 |
| Gene Full Name | gamma-aminobutyric acid type B receptor subunit 2 |
| Gene Summary | The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010] |
| Alternative Names | BcDNA:GH07312 antibody CG6706 antibody CT20836 antibody D Gaba2 antibody FLJ36928 antibody G protein coupled receptor 51 antibody G-protein coupled receptor 51 antibody GAB B R2 antibody GABA B R2 antibody GABA B receptor 2 antibody BcDNA:GH07312 antibody CG6706 antibody CT20836 antibody D Gaba2 antibody FLJ36928 antibody G protein coupled receptor 51 antibody G-protein coupled receptor 51 antibody GAB B R2 antibody GABA B R2 antibody GABA B receptor 2 antibody GABA-B receptor 2 antibody GABA-B-R2 antibody GABA-BR2 antibody GABAB R2 antibody GABABR 2 antibody GABABR2 antibody GABB R2 antibody GABBR 2 antibody Gabbr2 antibody GABR2_HUMAN antibody Gamma aminobutyric acid B receptor 2 antibody Gamma aminobutyric acid GABA B receptor 2 antibody Gamma aminobutyric acid type B receptor subunit 2 antibody Gamma-aminobutyric acid type B receptor subunit 2 antibody Gb 2 antibody Gb2 antibody GH07312 antibody GPR 51 antibody GPR51 antibody GPRC 3B antibody GPRC3B antibody HG 20 antibody HG20 antibody HRIHFB2099 antibody Metabotropic GABA B receptor subtype 2 antibody OTTHUMP00000021776 antibody OTTHUMP00000063797 antibody R2 SUBUNIT antibody |
| Molecular Weight(MW) | Predicted band size: 106 kDa |
| Cellular Localization | Plasma membrane |
WB
Western blot analysis of GABA B Receptor 2 on different lysates with Rabbit anti-GABA B Receptor 2 antibody at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti- GABA B Receptor 2 antibody. Counter stained with hematoxylin.
IF-P
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 2 with Rabbit anti-GABA B Receptor 2 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody( green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
FC
Flow cytometric analysis of SH-SY-5Y cells with GABA B Receptor 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Application Notes | WB:1:1000 IHC:1:50-1:200 IF-P:1:50 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
