WB
Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IHC
Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.ICC/IF
Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400)(Green). DAPI was used for nuclear staining (blue). objective: 100x.IP
Immunoprecipitation analysis of 300ug extracts of 293T cells using 3 ug HuR/ELAVL1 antibody). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody at a dilution of 1:1000.| Product Name | [KO Validated] HuR/ELAVL1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ELAVL1 |
|---|---|
| Gene Synonyms | HUR Hua MelG ELAV1 |
| Gene Full Name | ELAV like RNA binding protein 1 |
| Gene Summary | The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy. [provided by RefSeq, Sep 2012] |
| Alternative Names | HUR Hua MelG ELAV1 L1 |
| Molecular Weight(MW) | Predicted band size: 36 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IHC
Immunohistochemistry analysis of HuR/ELAVL1 in paraffin-embedded human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ICC/IF
Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400)(Green). DAPI was used for nuclear staining (blue). objective: 100x.
IP
Immunoprecipitation analysis of 300ug extracts of 293T cells using 3 ug HuR/ELAVL1 antibody). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody at a dilution of 1:1000.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
