WB
Western blot analysis of various lysates, using MUC2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma using MUC2 Rabbit pAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Immunofluorescence analysis of Human colorectal carcinoma using MUC2 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat AntiRabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Product Name | MUC2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 850-1270 of human MUC2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | AFCIF-PIHCWB |
| WB:1:500-1:1000 IHC::1:500-1:1000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MUC2 |
|---|---|
| Gene Synonyms | MLP SMUC MUC-2 |
| Gene Full Name | mucin 2, oligomeric mucus/gel-forming |
| Gene Summary | This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis. [provided by RefSeq, Oct 2016] |
| Alternative Names | MLP SMUC MUC-2 MUC2 |
| Molecular Weight(MW) | 551kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of various lysates, using MUC2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma using MUC2 Rabbit pAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Immunofluorescence analysis of Human colorectal carcinoma using MUC2 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat AntiRabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:500-1:1000 IHC::1:500-1:1000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | : PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
