WB
Western Blot analysis of various,using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000| Product Name | Smad1/5/9 (Phospho Ser463+Ser465) rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from human Smad1/5/9 (Phospho-Ser463+Ser465) |
| Modification | p-Ser463+Ser465 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000 |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Smad1/5/9 |
|---|---|
| Gene Synonyms | BSP1 JV41 BSP-1 JV4-1 MADH1 MADR1 |
| Gene Full Name | SMAD family member 1/5/9 |
| Gene Summary | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 51kDa |
| Cellular Localization | Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity). . |
WB
Western Blot analysis of various,using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000| Application Notes | WB:1:500-2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
