WB
Western blot analysis of extracts of various cell lines, using MARCO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.ICC/IF
Immunofluorescence analysis of rat lung cells using MARCO Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.| Product Name | MARCO Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 421-520 of human MARCO (NP_006761.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | MARCO |
|---|---|
| Gene Synonyms | SR-A6 SCARA2 |
| Gene Full Name | macrophage receptor with collagenous structure |
| Gene Summary | The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 43kDa/52kDa |
WB
Western blot analysis of extracts of various cell lines, using MARCO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
ICC/IF
Immunofluorescence analysis of rat lung cells using MARCO Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
