WB
Western Blot analysis of K562 cells using Glut1 Polyclonal Antibody diluted at 1:500IHC
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Glut1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of Hela cell. 1,Glut1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.| Product Name | Glut1 Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GLUT1. AA range:441-490 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC2A1 |
|---|---|
| Gene Synonyms | CSE PED DYT9 GLUT DYT17 DYT18 EIG12 GLUT1 HTLVR GLUT-1 SDCHCN GLUT1DS |
| Gene Full Name | solute carrier family 2 member 1 |
| Gene Summary | This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013] |
| Molecular Weight(MW) | 55kDa |
| Source | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Cellular Localization | Cell membrane ; Multi-pass membrane protein . Melanosome . Photoreceptor inner segment . Localizes primarily at the cell surface (PubMed:18245775, PubMed:19449892, PubMed:23219802, PubMed:25982116, PubMed:24847886). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). . |
WB
Western Blot analysis of K562 cells using Glut1 Polyclonal Antibody diluted at 1:500
IHC
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Glut1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,Glut1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
