WB
Western blot analysis of FOXI1 using anti-FOXI1 antibody.The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysates,Lane 2: monkey COS-7 cell lysates.Use rabbit anti-FOXI1 1:1000, probed with a goat anti-rabbit IgGHRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.A specific band was detected for FOXI1 at approximately 60KD. The expected band size for FOXI1 is at 41KD.ICC/IF
ICC analysis using anti-FOXI1 antibody was detected in immersion fixed U2OS cell line.Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody(green)and counterstained with DAPI (blue).FCM
Flow Cytometry analysis of A549 cells using anti-FOXI1 antibody . Overlay histogram showing A549 cells stained with FOXI1 antibody (Blue line). To facilitate intracellular staining,cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXI1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-FOXI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human FOXI1 recombinant protein (Position: M1-V378). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCMICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FCM:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXI1 |
|---|---|
| Gene Synonyms | HFH3 FKH10 HFH-3 FKHL10 FREAC6 FREAC-6 |
| Gene Full Name | forkhead box I1 |
| Gene Summary | This gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene may play an important role in the development of the cochlea and vestibulum, as well as in embryogenesis. The encoded protein has been found to be required for the transcription of four subunits of a proton pump found in the inner ear, the kidney, and the epididymis. Mutations in this gene have been associated with deafness, autosomal recessive 4. [provided by RefSeq, Jan 2017] |
| Alternative Names | Forkhead box I1 FKH10 FKHL10 FREAC-6 FREAC6 HFH-3 HFH3 FOXI1 |
| Molecular Weight(MW) | 41kDa(Observed MW 60kDa) |
WB
Western blot analysis of FOXI1 using anti-FOXI1 antibody.The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysates,Lane 2: monkey COS-7 cell lysates.Use rabbit anti-FOXI1 1:1000, probed with a goat anti-rabbit IgGHRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.A specific band was detected for FOXI1 at approximately 60KD. The expected band size for FOXI1 is at 41KD.
ICC/IF
ICC analysis using anti-FOXI1 antibody was detected in immersion fixed U2OS cell line.Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody(green)and counterstained with DAPI (blue).
FCM
Flow Cytometry analysis of A549 cells using anti-FOXI1 antibody . Overlay histogram showing A549 cells stained with FOXI1 antibody (Blue line). To facilitate intracellular staining,cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXI1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FCM:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
