FC
Flow cytometric analysis of 293T cells with TWEAKR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TWEAKR antibody. Counter stained with hematoxylin.ICC/IF
ICC staining TWEAKR in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | TWEAKR Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human TWEAKR aa 80-129 / 129. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHC |
| FC:1:50-1:100 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNFRSF12A |
|---|---|
| Gene Synonyms | FN14 CD266 TWEAKR |
| Gene Full Name | TNF receptor superfamily member 12A |
| Gene Summary | Involved in positive regulation of extrinsic apoptotic signaling pathway and regulation of wound healing. Predicted to be located in cell surface and ruffle. Predicted to be active in plasma membrane. [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | CD 266 antibody CD266 antibody CD266 antigen antibody FGF inducible 14 antibody FGF-inducible 14 antibody Fibroblast growth factor inducible immediate early response protein 14 antibody Fibroblast growth factor-inducible immediate-early response protein 14 antibody FN 14 antibody FN14 antibody TNFRSF 12A antibody CD 266 antibody CD266 antibody CD266 antigen antibody FGF inducible 14 antibody FGF-inducible 14 antibody Fibroblast growth factor inducible immediate early response protein 14 antibody Fibroblast growth factor-inducible immediate-early response protein 14 antibody FN 14 antibody FN14 antibody TNFRSF 12A antibody TNFRSF12A antibody TNR12_HUMAN antibody Tumor necrosis factor receptor superfamily member 12A antibody TWEAK R antibody Tweak receptor antibody Tweak-receptor antibody TweakR antibody |
| Cellular Localization | Membrane. |
FC
Flow cytometric analysis of 293T cells with TWEAKR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TWEAKR antibody. Counter stained with hematoxylin.
ICC/IF
ICC staining TWEAKR in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Application Notes | FC:1:50-1:100 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
