WB
Western blot analysis of Myeloperoxidase on different lysates with Rabbit anti-Myeloperoxidase antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (negative) Lane 2: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84/55 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Myeloperoxidase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of Jurkat cells labeling Myeloperoxidase with Rabbit anti-Myeloperoxidase antibody at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myeloperoxidase antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Species: Human, Site: tonsil, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.| Product Name | Myeloperoxidase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Myeloperoxidase aa 600-700 (C terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:5000 IHC:1:1000 ICC/IF:1:100 IF-P:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MPO |
|---|---|
| Gene Full Name | myeloperoxidase |
| Gene Summary | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Alternative Names | 84 kDa myeloperoxidase antibody 89 kDa myeloperoxidase antibody EC 1.11.1.7 antibody EC1.11.2.2 antibody fj80f04 antibody MPO antibody mpx antibody myeloid-specific peroxidase antibody Myeloperoxidase antibody Myeloperoxidase heavy chain antibody 84 kDa myeloperoxidase antibody 89 kDa myeloperoxidase antibody EC 1.11.1.7 antibody EC1.11.2.2 antibody fj80f04 antibody MPO antibody mpx antibody myeloid-specific peroxidase antibody Myeloperoxidase antibody Myeloperoxidase heavy chain antibody Myeloperoxidase light chain antibody PERM_HUMAN antibody wu:fj80f04 antibody |
| Molecular Weight(MW) | 84 kDa(Observed band size: 84/55 kDa) |
| Cellular Localization | Lysosome. |
WB
Western blot analysis of Myeloperoxidase on different lysates with Rabbit anti-Myeloperoxidase antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate (negative) Lane 2: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84/55 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Myeloperoxidase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Jurkat cells labeling Myeloperoxidase with Rabbit anti-Myeloperoxidase antibody at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Myeloperoxidase antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Species: Human, Site: tonsil, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.| Application Notes | WB:1:5000 IHC:1:1000 ICC/IF:1:100 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
