WB
Variouslysates were subjected to SDS PAGE followed by western blot with TGM1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffinembedded human cervical cancertissue slide using TGM1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).IP
IP result of anti-TGM1 (IP:4ug; Detection:1:1000) with mouse kidney tissue lysate 4000ug.| Product Name | TGM1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | TGM1 fusion protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:8000 IHC:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Symbol | TGM1 |
|---|---|
| Gene Synonyms | LI KTG LI1 TGK ICR2 ARCI1 TGASE |
| Gene Full Name | transglutaminase 1 |
| Gene Summary | The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). [provided by RefSeq, Jul 2008] |
| Alternative Names | LI1 LI KTG ICR2 Epidermal TGase |
| Molecular Weight(MW) | 90kDa |
| Cellular Localization | Membrane |
WB
Variouslysates were subjected to SDS PAGE followed by western blot with TGM1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffinembedded human cervical cancertissue slide using TGM1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).
IP
IP result of anti-TGM1 (IP:4ug; Detection:1:1000) with mouse kidney tissue lysate 4000ug.| Application Notes | WB:1:1000-1:8000 IHC:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
