WB
Western blot analysis of RASSF2 on different lysates with Rabbit anti-RASSF2 antibody at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | RASSF2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RASSF2 |
|---|---|
| Gene Synonyms | CENP-34 RASFADIN |
| Gene Full Name | Ras association domain family member 2 |
| Gene Summary | This gene encodes a protein that contains a Ras association domain. Similar to its cattle and sheep counterparts, this gene is located near the prion gene. Two alternatively spliced transcripts encoding the same isoform have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | CENP 34 antibody Centromere protein 34 antibody Ras association (RalGDS/AF 6) domain family 2 antibody Ras association (RalGDS/AF-6) domain family member 2 antibody Ras association domain containing protein 2 antibody Ras association domain-containing protein 2 antibody RASF2_HUMAN antibody RASFADIN antibody RASSF2 antibody RP4-599I11.1 antibody |
| Molecular Weight(MW) | 38kDa |
| Cellular Localization | Nucleus, Cytoplasm, Chromosome, centromere, kinetochore. |
WB
Western blot analysis of RASSF2 on different lysates with Rabbit anti-RASSF2 antibody at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
