WB
Western blot analysis of extracts from various samples, using DDR1 Ab. Lane 1: Rat kidney, blocked with antigen-specific peptides. Lane 2: Rat kidney. Lane 3: Mouse liver. Lane 4: Mouse lung.IHC
DDR1 Ab at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
DDR1 Ab staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to DDR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human DDR1, corresponding to a region within C-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDR1 |
|---|---|
| Gene Synonyms | CAK DDR NEP HGK2 PTK3 RTK6 TRKE CD167 EDDR1 MCK10 NTRK4 PTK3A |
| Gene Full Name | discoidin domain receptor tyrosine kinase 1 |
| Gene Summary | Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011] |
| Alternative Names | CAK CD 167 CD167 CD167 antigen-like family member A CD167a Cell adhesion kinase DDR 1 DDR DDR1 DDR1_HUMAN Discoidin domain receptor Discoidin domain receptor tyrosine kinase 1 Discoidin receptor tyrosine kinase Discoidin receptor tyrosine kinase isoform a EDDR 1 EDDR1 Epithelial discoidin domain receptor 1 Epithelial discoidin domain-containing receptor 1 Epithelial specific receptor kinase HGK2 Mammarian carcinoma kinase 10 Mammary carcinoma kinase 10 MCK-10 MCK10 NEP Neuroepithelial tyrosine kinase Neurotrophic tyrosine kinase receptor type 4 NTRK 4 NTRK4 OTTHUMP00000029343 OTTHUMP00000029344 OTTHUMP00000029345 OTTHUMP00000029346 OTTHUMP00000029347 OTTHUMP00000164863 OTTHUMP00000164867 OTTHUMP00000222080 Protein-tyrosine kinase 3A Protein-tyrosine kinase RTK-6 PTK 3 PTK 3A protein tyrosine kinase 3A PTK3 PTK3A Receptor tyrosine kinase NEP RTK 6 RTK6 TRK E TRKE Tyrosine kinase DDR Tyrosine kinase receptor E Tyrosine-protein kinase CAK |
| Molecular Weight(MW) | 110kDa; 101kDa(Calculated). |
| Cellular Localization | Cell membrane,Secreted |
WB
Western blot analysis of extracts from various samples, using DDR1 Ab. Lane 1: Rat kidney, blocked with antigen-specific peptides. Lane 2: Rat kidney. Lane 3: Mouse liver. Lane 4: Mouse lung.
IHC
DDR1 Ab at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
DDR1 Ab staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
