WB
Western blot analysis of Claudin 1 on different lysates with Rabbit anti-Claudin 1 antibody at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HUVEC cell lysate (20 µg/Lane) Lane 3: Caco-2 cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse skin tissue lysate (40 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Rat skin tissue lysate (40 µg/Lane) Lane 8: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 19 kDa Exposure time: Lane 1-2: 8 seconds; Lane 3-8: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Claudin 1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Claudin 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Claudin 1 aa 164-211 / 211. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN1 |
|---|---|
| Gene Synonyms | CLD1 SEMP1 ILVASC |
| Gene Full Name | claudin 1 |
| Gene Summary | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008] |
| Alternative Names | Claudin-1 antibody Claudin1 antibody CLD 1 antibody CLD1 antibody CLD1_HUMAN antibody CLDN 1 antibody Cldn1 antibody ILVASC antibody SEMP 1 antibody SEMP1 antibody Claudin-1 antibody Claudin1 antibody CLD 1 antibody CLD1 antibody CLD1_HUMAN antibody CLDN 1 antibody Cldn1 antibody ILVASC antibody SEMP 1 antibody SEMP1 antibody Senescence associated epithelial membrane protein 1 antibody Senescence associated epithelial membrane protein antibody Senescence-associated epithelial membrane protein antibody |
| Molecular Weight(MW) | 23kDa(Observed band size: 19 kDa) |
| Cellular Localization | Cell junction, tight junction, Cell membrane, Basolateral cell membrane. |
WB
Western blot analysis of Claudin 1 on different lysates with Rabbit anti-Claudin 1 antibody at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HUVEC cell lysate (20 µg/Lane) Lane 3: Caco-2 cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse skin tissue lysate (40 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Rat skin tissue lysate (40 µg/Lane) Lane 8: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 19 kDa Exposure time: Lane 1-2: 8 seconds; Lane 3-8: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Claudin 1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
