WB
Western blot analysis of extracts from various samples, using Phospho-PDHA1/2 (Ser293/Ser291) Antibody. Lane 1: Pc12 cells(heat-shock treatment), blocked with antigen-specific peptides. Lane 2: Pc12 cells(heat-shock treatment). Lane 3: 3t3-l1 cells(heat-shock treatment). Lane 4: Hepg2 cells(heat-shock treatment). Lane 5: Hela cells(heat-shock treatment).IHC
Rabbit polyclonal antibody to Phospho-PDHA1/2 (Ser293/Ser291) at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
Rabbit polyclonal antibody to Phospho-PDHA1/2 (Ser293/Ser291) staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Phospho-PDHA1/2 (Ser293/Ser291) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human PDHA1(Accession P08559), corresponding to amino acid residues around phosphorylated Ser293. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDHA1 |
|---|---|
| Gene Synonyms | PDHA PDHAD PHE1A E1alpha PDHCE1A |
| Gene Full Name | pyruvate dehydrogenase E1 subunit alpha 1 |
| Gene Summary | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] |
| Molecular Weight(MW) | 43kDa |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of extracts from various samples, using Phospho-PDHA1/2 (Ser293/Ser291) Antibody. Lane 1: Pc12 cells(heat-shock treatment), blocked with antigen-specific peptides. Lane 2: Pc12 cells(heat-shock treatment). Lane 3: 3t3-l1 cells(heat-shock treatment). Lane 4: Hepg2 cells(heat-shock treatment). Lane 5: Hela cells(heat-shock treatment).
IHC
Rabbit polyclonal antibody to Phospho-PDHA1/2 (Ser293/Ser291) at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
Rabbit polyclonal antibody to Phospho-PDHA1/2 (Ser293/Ser291) staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM643077