WB
10 % fetal bovine serum treated HeLa cells and Serum-free treated HeLa cells were subjected to SDS PAGE followed by western blot with Phospho-PDH E1 Alpha (Ser300) antibody at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Product Name | Phospho-PDH E1 Alpha (Ser300) Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:14000 ICC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 450ug/ml |
| Purification | Affinity purified |
| Gene Symbol | PDHA1 |
|---|---|
| Gene Synonyms | PDHA PDHAD PHE1A E1alpha PDHCE1A |
| Gene Full Name | pyruvate dehydrogenase E1 subunit alpha 1 |
| Gene Summary | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] |
| Molecular Weight(MW) | 43 kDa(Observed Molecular Weight 40 kDa) |
| Cellular Localization | Mitochondrion matrix. |
WB
10 % fetal bovine serum treated HeLa cells and Serum-free treated HeLa cells were subjected to SDS PAGE followed by western blot with Phospho-PDH E1 Alpha (Ser300) antibody at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:2000-1:14000 ICC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643079