WB
Western blot analysis of Sodium Potassium ATPase on different lysates with Rabbit anti-Sodium Potassium ATPase antibody at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Immunofluorescence analysis of paraffin-embedded rat kidney tissue labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human ATP1A1 aa 39-83 / 1023. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:50000-1:100000 IHC:1:2000-1:5000 ICC:1:100-1:500 IF-P:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATP1A1 |
|---|---|
| Gene Synonyms | CMT2DD HOMGSMR2 |
| Gene Full Name | ATPase Na+/K+ transporting subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Alternative Names | ATPase Na+/K+ transporting alpha antibody adenosinetriphosphatase antibody AT1A1_HUMAN antibody ATP1A1 antibody ATP1A4 antibody ATP1AL2 antibody ATP1B antibody ATP1B1 antibody ATPase Na+/K+ transporting alpha 1 polypeptide antibody ATPase Na+/K+ transporting alpha 4 polypeptide antibody ATPase Na+/K+ transporting alpha antibody adenosinetriphosphatase antibody AT1A1_HUMAN antibody ATP1A1 antibody ATP1A4 antibody ATP1AL2 antibody ATP1B antibody ATP1B1 antibody ATPase Na+/K+ transporting alpha 1 polypeptide antibody ATPase Na+/K+ transporting alpha 4 polypeptide antibody ATPase Na+/K+ transporting beta 1 polypeptide antibody ATPase Na+/K+ transporting alpha polypeptide-like 2 antibody ATPase Na+/K+ transporting beta 1 polypeptide antibody Beta 1-subunit of Na(+) K(+)-ATPase antibody Na(+)/K(+) ATPase alpha-1 subunit antibody Na(+)/K(+) ATPase alpha-4 subunit antibody Na+ K+ ATPase alpha subunit antibody Na+/K+ ATPase 1 antibody Na+/K+ ATPase 4 antibody Na+/K+ ATPase alpha-D polypeptide antibody Na K-ATPase beta-1 polypeptide antibody Na K-ATPase alpha-A catalytic polypeptide antibody Na K-ATPase catalytic subunit alpha-A protein antibody Na K-ATPase subunit alpha-C antibody polypeptide-like 2 antibody Sodium pump 1 antibody sodium pump 4 antibody Sodium pump subunit alpha-1 antibody sodium pump subunit alpha-4 antibody sodium pump subunit beta-1 antibody sodium-potassium ATPase catalytic subunit alpha-1 antibody sodium-potassium ATPase catalytic subunit alpha-4 antibody sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody sodium-potassium ATPase alpha 4 polypeptide antibody sodium-potassium-ATPase alpha 1 polypeptide antibody Sodium/potassium transporting ATPase alpha 1 chain antibody Sodium/potassium transporting ATPase subunit beta 1 antibody sodium/potassium-dependent ATPase beta-1 subunit antibody sodium/potassium-transporting ATPase alpha-4 chain antibody sodium/potassium-transporting ATPase beta-1 chain antibody Sodium/potassium-transporting ATPase subunit alpha-1 antibody sodium/potassium-transporting ATPase subunit alpha-4 antibody |
| Molecular Weight(MW) | 113kDa(Observed band size: 100kDa) |
| Cellular Localization | Cell membrane, Melanosome. |
WB
Western blot analysis of Sodium Potassium ATPase on different lysates with Rabbit anti-Sodium Potassium ATPase antibody at 1/100,000 dilution. Lane 1: HeLa cell lysate (no heat) (20 µg/Lane) Lane 2: HT-29 cell lysate (no heat) (20 µg/Lane) Lane 3: HepG2 cell lysate (no heat) (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (no heat) (20 µg/Lane) Lane 5: L-929 cell lysate (no heat) (20 µg/Lane) Lane 6: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 7: Rat brain tissue lysate (no heat) (20 µg/Lane) Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Sodium Potassium ATPase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Immunofluorescence analysis of paraffin-embedded rat kidney tissue labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:50000-1:100000 IHC:1:2000-1:5000 ICC:1:100-1:500 IF-P:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
