WB
Western blot analysis of KDM1 / LSD1 on different lysates with Rabbit anti-KDM1 / LSD1 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: COS-1 cell lysate (20 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 93 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KDM1 / LSD1 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of Jurkat cells labeling KDM1 / LSD1 with Rabbit anti-KDM1 / LSD1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KDM1 / LSD1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.IP
KDM1 / LSD1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with first antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using first antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: KDM1 / LSD1 antibody IP in Jurkat cell lysate Lane 3: Rabbit IgG instead ofKDM1 / LSD1 antibody in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 44 seconds.| Product Name | KDM1 / LSD1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000 IHC:1:50-1:200 ICC:1:100 IP:1-2ug/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KDM1A |
|---|---|
| Gene Synonyms | AOF2 CPRF KDM1 LSD1 AIMAH3 BHC110 |
| Gene Full Name | lysine demethylase 1A |
| Gene Summary | This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| Alternative Names | Amine oxidase (flavin containing) domain 2 antibody Amine oxidase flavin containing 2 antibody AOF2 antibody BHC110 antibody BRAF35 HDAC complex protein BHC110 antibody BRAF35-HDAC complex protein BHC110 antibody BRAF35/HDAC complex 110-kD subunit antibody CPRF antibody EC1 antibody FAD binding protein BRAF35 HDAC complex 110 kDa subunit antibody Amine oxidase (flavin containing) domain 2 antibody Amine oxidase flavin containing 2 antibody AOF2 antibody BHC110 antibody BRAF35 HDAC complex protein BHC110 antibody BRAF35-HDAC complex protein BHC110 antibody BRAF35/HDAC complex 110-kD subunit antibody CPRF antibody EC1 antibody FAD binding protein BRAF35 HDAC complex 110 kDa subunit antibody Flavin-containing amine oxidase domain-containing protein 2 antibody KDM 1 antibody KDM1 antibody Kdm1a antibody KDM1A_HUMAN antibody KIAA0601 antibody LSD 1 antibody LSD1 antibody Lysine (K) specific demethylase 1 antibody Lysine (K) specific demethylase 1A antibody Lysine demethylase 1A antibody Lysine specific histone demethylase 1 antibody Lysine specific histone demethylase 1A antibody Lysine-specific demethylase 1 antibody Lysine-specific demethylase 1A antibody Lysine-specific histone demethylase 1A antibody |
| Molecular Weight(MW) | 93kDa(Observed band size: 110kDa) |
| Cellular Localization | Nucleus, Chromosome. |
WB
Western blot analysis of KDM1 / LSD1 on different lysates with Rabbit anti-KDM1 / LSD1 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: COS-1 cell lysate (20 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 93 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KDM1 / LSD1 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Jurkat cells labeling KDM1 / LSD1 with Rabbit anti-KDM1 / LSD1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KDM1 / LSD1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IP
KDM1 / LSD1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with first antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using first antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: KDM1 / LSD1 antibody IP in Jurkat cell lysate Lane 3: Rabbit IgG instead ofKDM1 / LSD1 antibody in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 44 seconds.| Application Notes | WB:1:1000 IHC:1:50-1:200 ICC:1:100 IP:1-2ug/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
