WB
Western blot analysis of RCC2 using anti-RCC2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RCC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for RCC2 at approximately 56 kDa. The expected band size for RCC2 is at 56 kDa.ICC/IF
IF analysis of RCC2 using anti-RCC2 antibody. RCC2 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-RCC2 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to RCC2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human RCC2 recombinant protein (Position: K293-L522). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC:1:50-1:400 |
|
| Species Reactivity | Human |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Symbol | RCC2 |
|---|---|
| Gene Synonyms | TD-60 |
| Gene Full Name | regulator of chromosome condensation 2 |
| Gene Summary | The protein encoded by this gene is a guanine exchange factor that is active on RalA, a small GTPase. The encoded protein and RalA are both essential for proper kinetochore-microtubule function in early mitosis. This protein has been shown to be a biomarker for colorectal cancer. [provided by RefSeq, Oct 2016] |
| Alternative Names | KIAA1470 Protein RCC2 RCC1 like protein TD 60 RCC2 TD 60 TD60 |
| Molecular Weight(MW) | 56kDa |
| Cellular Localization | Nucleus, nucleolus,Cytoplasm, cytoskeleton,Cell membrane. |
WB
Western blot analysis of RCC2 using anti-RCC2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RCC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for RCC2 at approximately 56 kDa. The expected band size for RCC2 is at 56 kDa.
ICC/IF
IF analysis of RCC2 using anti-RCC2 antibody. RCC2 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-RCC2 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 ICC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
