WB
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MDA-MB-468 cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: HUVEC cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: Jurkat cell lysate (20 µg/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 µg/Lane) Lane 9: COS-1 cell lysate (20 µg/Lane) Lane 10: Neuro-2a cell lysate (20 µg/Lane) Lane 11: NIH/3T3 cell lysate (20 µg/Lane) Lane 12: C6 cell lysate (20 µg/Lane) Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HUVEC cells labeling Merlin with Rabbit anti-Merlin antibody at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Merlin antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of NIH/3T3 cells labeling Merlin. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Merlin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Merlin aa 1-350 / 595 (P35240). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:2000 ICC:1:100 FC:1:1000 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NF2 |
|---|---|
| Gene Synonyms | ACN SCH BANF SWNV merlin-1 |
| Gene Full Name | NF2, moesin-ezrin-radixin like (MERLIN) tumor suppressor |
| Gene Summary | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. [provided by RefSeq, May 2022] |
| Alternative Names | ACN antibody BANF antibody Bilateral acoustic neuroma antibody MERL_HUMAN antibody Merlin antibody Moesin ezrin radixin like protein antibody Moesin ezrin radizin like antibody Moesin-ezrin-radixin-like protein antibody Neurofibromatosis 2 antibody Neurofibromatosis type 2 antibody ACN antibody BANF antibody Bilateral acoustic neuroma antibody MERL_HUMAN antibody Merlin antibody Moesin ezrin radixin like protein antibody Moesin ezrin radizin like antibody Moesin-ezrin-radixin-like protein antibody Neurofibromatosis 2 antibody Neurofibromatosis type 2 antibody Neurofibromatosis2 antibody Neurofibromin 2 antibody Neurofibromin-2 antibody Neurofibromin2 antibody NF 2 antibody Nf2 antibody SCH antibody Schwannomerlin antibody Schwannomin antibody |
| Molecular Weight(MW) | 70kDa |
| Cellular Localization | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus. |
WB
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody at 1/2,000 dilution. Lane 1: PC-3M cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MDA-MB-468 cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: HUVEC cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: Jurkat cell lysate (20 µg/Lane) Lane 8: MDA-MB-231 cell lysate (negative) (20 µg/Lane) Lane 9: COS-1 cell lysate (20 µg/Lane) Lane 10: Neuro-2a cell lysate (20 µg/Lane) Lane 11: NIH/3T3 cell lysate (20 µg/Lane) Lane 12: C6 cell lysate (20 µg/Lane) Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HUVEC cells labeling Merlin with Rabbit anti-Merlin antibody at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Merlin antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of NIH/3T3 cells labeling Merlin. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 ICC:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
