WB
Western blot analysis of various lysates, using Acetyl-Histone H4-K12 Rabbit mAb at 1:1000 dilution. HeLa cells were treated by TSA (1uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Acetyl-Histone H4-K12 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.DB
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K12 antibody at 1:1000 dilution.IHC
Immunohistochemistry analysis of paraffin embedded Mouse intestin tissue using Acetyl-Histone H4-K12 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen tissue using Acetyl Histone H4-K12 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K12 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Acetyl-Histone H4-K12 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic acetylated peptide around K12 of human Histone H4（P62805）. |
| Modification | Acetyl K12 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:500 DB:1:500-1:1000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | AllHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4FA H4-16 H4C11 H4C12 H4C13 H4C14 H4C15 H4C16 HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Alternative Names | H4 H4/n H4C1 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4F2 H4FN FO108 H4-16 H4C11 H4C12 H4C13 H4C15 H4C16 HIST2H4 HIST2H4A Acetyl-Histone H4-K12 |
| Molecular Weight(MW) | 11kDa( Observed MW 15kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates, using Acetyl-Histone H4-K12 Rabbit mAb at 1:1000 dilution. HeLa cells were treated by TSA (1uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Acetyl-Histone H4-K12 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
DB
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K12 antibody at 1:1000 dilution.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse intestin tissue using Acetyl-Histone H4-K12 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen tissue using Acetyl Histone H4-K12 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K12 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 DB:1:500-1:1000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
