Welcome to Omnimabs!
TEL: 02154156726 Info: info@Omnimabs.com CN
Cleaved Caspase-3 Recombinant Rabbit Monoclonal Antibody
Catalog Number:OM643190
OM643190
Discount Price
OM643190-100ul
2~3 Week
100ul
OM643190-50ul
50ul
OM643190-20ul
20ul
2~3 Week
Product Profile
Product Name Cleaved Caspase-3 Recombinant Rabbit Monoclonal Antibody
Antibody Type Primary Antibodies
Immunogen Synthetic peptide within Human Caspase-3 aa 28-67 / 277.
Key Feature
Clonality monoclonal
Isotype IgG
Host Species Rabbit
Tested Applications ICC/IFIHCWB
WB:1:1000-1:2000
IHC:1:50-1:200
ICC:1:50-1:200
Species Reactivity HumanPig
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol CASP3
Gene Synonyms CPP32
SCA-1
CPP32B
Gene Full Name caspase 3
Gene Summary The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]
Molecular Weight(MW) 17kDa
Cellular Localization Cytoplasm
Application

WB

Western blot analysis of Active Caspase-3 on different lysates with Rabbit anti-Active Caspase-3 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 25μM Etoposide for 5 hours cell lysate Lane 3: Jurkat treated with 25μM Etoposide for 16 hours cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

IHC

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Active Caspase-3 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ICC/IF

ICC staining of Active Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application Notes WB:1:1000-1:2000
IHC:1:50-1:200
ICC:1:50-1:200
Additional Information
Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
validation
question title
question detaiil
Verification code
 

References(0)