WB
Western blot analysis of various lysates using Lipocalin-2/NGAL Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): 293T Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Lipocalin-2/NGAL Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embedded Human spleen tissue using Lipocalin-2/NGAL Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. objective: 40x.| Product Name | Lipocalin-2/NGAL Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of human/mouse Lipocalin-2/NGAL. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:500-1:2000 IF-P:1:200-1:400 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LCN2 |
|---|---|
| Gene Synonyms | p25 24p3 MSFI NGAL |
| Gene Full Name | lipocalin 2 |
| Gene Summary | This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015] |
| Alternative Names | p25 24p3 MSFI NGAL Lipocalin-2/NGAL AW212229 NRL Sip24 |
| Molecular Weight(MW) | 23kDa |
| Cellular Localization | Secreted, Cytoplasmic granule lumen, Cytoplasmic vesicle lumen. |
WB
Western blot analysis of various lysates using Lipocalin-2/NGAL Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): 293T Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Lipocalin-2/NGAL Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embedded Human spleen tissue using Lipocalin-2/NGAL Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. objective: 40x.| Application Notes | WB:1:1000-1:5000 IHC:1:500-1:2000 IF-P:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3. |
