WB
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at 1:500 dilution.NIH/3T3 cells were treated by TSA (1uM) at 37℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.IHC
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.| Product Name | Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic acetylated peptide around K5/K8/K12/K16 of human Histone H4. |
| Modification | Acetyl K12/K16 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4FA H4-16 H4C11 H4C12 H4C13 H4C14 H4C15 H4C16 HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Alternative Names | H4 H4/n H4C1 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4F2 H4FN FO108 H4-16 H4C11 H4C12 H4C13 H4C15 H4C16 HIST2H4 HIST2H4A Acetyl-Histone H4-K5/K8/K12/K16 |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | extracellular exosome,extracellular region,nucleoplasm,nucleus |
WB
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at 1:500 dilution.NIH/3T3 cells were treated by TSA (1uM) at 37℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.| Application Notes | WB:1:100-1:500 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
