WB
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HCT 116 cell lysate Lane 5: HepG2 cell lysate Lane 6: SK-MEL-28 cell lysate Lane 7: A375 cell lysate Lane 8: B16-F1 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining of CD9 in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).FC
Flow cytometric analysis of B16-F1 cells labeling CD9. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | CD9 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CD9 aa 179-228 / 228. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:200-1:1000 ICC:1:50 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD9 |
|---|---|
| Gene Synonyms | MIC3 MRP-1 BTCC-1 DRAP-27 TSPAN29 TSPAN-29 |
| Gene Full Name | CD9 molecule |
| Gene Summary | This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011] |
| Alternative Names | Tetraspanin 29 antibody 5H9 antibody 5H9 antigen antibody Antigen defined by monoclonal antibody 602 29 antibody Antigen defined by monoclonal antibody 60229 antibody BA-2/p24 antigen antibody BA2 antibody BTCC 1 antibody Tetraspanin 29 antibody 5H9 antibody 5H9 antigen antibody Antigen defined by monoclonal antibody 602 29 antibody Antigen defined by monoclonal antibody 60229 antibody BA-2/p24 antigen antibody BA2 antibody BTCC 1 antibody BTCC1 antibody CD9 antibody CD9 antigen antibody CD9 antigen p24 antibody CD9 molecule antibody CD9_HUMAN antibody Cell growth inhibiting gene 2 protein antibody Cell growth-inhibiting gene 2 protein antibody DRAP 27 antibody DRAP27 antibody GIG2 antibody Growth inhibiting gene 2 protein antibody Leukocyte antigen MIC3 antibody MIC3 antibody Motility related protein antibody Motility-related protein antibody MRP 1 antibody MRP-1 antibody MRP1 antibody p24 antibody p24 antigen antibody Tetraspanin-29 antibody Tspan 29 antibody Tspan-29 antibody TSPAN29 antibody |
| Molecular Weight(MW) | 25kDa(Observed band size: 23kDa) |
| Cellular Localization | Cell membrane, Membrane |
WB
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HCT 116 cell lysate Lane 5: HepG2 cell lysate Lane 6: SK-MEL-28 cell lysate Lane 7: A375 cell lysate Lane 8: B16-F1 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of CD9 in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
FC
Flow cytometric analysis of B16-F1 cells labeling CD9. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000-1:2000 IHC:1:200-1:1000 ICC:1:50 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
