WB
Western blot analysis of Histone H4 (acetyl K5) on different lysates with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lane 4: C6 cell lysate Lane 5: C6 treated with 1μM TSA for 18 hours cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.IF-P
Immunofluorescence staining of paraffin- embedded rat brain tissue using anti-Histone H4(acetyl K5) antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/300 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.| Product Name | Histone H4 (acetyl K5) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Histone H4 aa 1-50 / 103 (acetyl K5). |
| Modification | acetyl K5 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1,000-1:5,000 IHC:1:200-1:1000 ICC:1:100-1:500 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4FA H4-16 H4C11 H4C12 H4C13 H4C14 H4C15 H4C16 HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Alternative Names | Histone gene cluster 1 H4A antibody Histone gene cluster 2 H4 antibody dJ160A22.1 antibody dJ160A22.2 antibody dJ221C16.1 antibody dJ221C16.9 antibody FO108 antibody H4 antibody H4 histone family member A antibody H4 histone family member B antibody Histone gene cluster 1 H4A antibody Histone gene cluster 2 H4 antibody dJ160A22.1 antibody dJ160A22.2 antibody dJ221C16.1 antibody dJ221C16.9 antibody FO108 antibody H4 antibody H4 histone family member A antibody H4 histone family member B antibody H4 histone family member C antibody H4 histone family member D antibody H4 histone family member E antibody H4 histone family member G antibody H4 histone family member H antibody H4 histone family member I antibody H4 histone family member J antibody H4 histone family member K antibody H4 histone family member M antibody H4 histone family member N antibody H4 histone family 2 antibody H4/A antibody H4/B antibody H4/C antibody H4/D antibody H4/E antibody H4/G antibody H4/H antibody H4/I antibody H4/J antibody H4/K antibody H4/M antibody H4/N antibody H4/O antibody H4/p antibody H4_HUMAN antibody H4F2 antibody H4F2iii antibody H4F2iv antibody H4FA antibody H4FB antibody H4FC antibody H4FD antibody H4FE antibody H4FG antibody H4FH antibody H4FI antibody H4FJ antibody H4FK antibody HIST1 cluster H4A antibody HIST1 cluster H4B antibody HIST1 cluster H4D antibody HIST2H4 antibody Hist4 cluster H4 antibody Hist4h4 antibody histone 1 H4a antibody histone 1 H4c antibody histone 1 H4d antibody histone 1 H4f antibody histone 1 H4h antibody histone 1 H4i antibody histone 1 H4j antibody histone 1 H4k antibody histone 1 H4l antibody histone 2 H4a antibody histone 2 H4b antibody Histone 4 family member M antibody histone 4 H4 antibody histone cluster 1 H4 antibody histone cluster 1 H4a antibody histone cluster 1 H4b antibody histone cluster 1 H4c antibody histone cluster 1 H4d antibody histone cluster 1 H4e antibody histone cluster 1 H4f antibody histone cluster 1 H4h antibody histone cluster 1 H4i antibody histone cluster 1 H4j antibody histone cluster 1 H4k antibody histone cluster 1 H4l antibody histone cluster 2 H4a antibody histone cluster 2 H4b antibody histone cluster 4 H4 antibody Histone family member A antibody Histone family member B antibody Histone family member D antibody Histone family member H antibody Histone family member I antibody Histone family member L antibody Histone gene cluster 1 H4 antibody Histone gene cluster 1 H4D antibody Histone gene cluster 1 H4E antibody Histone gene cluster 1 H4K antibody Histone gene cluster 4 H4 antibody Histone gene cluster 4 H4 histone antibody Histone H4 antibody histone IV family 2 antibody H4K5ac antibody |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | Nucleus, Chromosome. |
WB
Western blot analysis of Histone H4 (acetyl K5) on different lysates with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lane 4: C6 cell lysate Lane 5: C6 treated with 1μM TSA for 18 hours cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Immunofluorescence staining of paraffin- embedded rat brain tissue using anti-Histone H4(acetyl K5) antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/300 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.| Application Notes | WB:1:1,000-1:5,000 IHC:1:200-1:1000 ICC:1:100-1:500 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
