WB
Western blot analysis of extracts from Rat brain, using Glycoprotein 2 Antibody.ICC/IF
Glycoprotein 2 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to Glycoprotein 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from Human Glycoprotein 2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC:1:100-1:500 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GP2 |
|---|---|
| Gene Synonyms | ZAP75 |
| Gene Full Name | glycoprotein 2 |
| Gene Summary | This gene encodes an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. The encoded protein binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| Alternative Names | DKFZp779K0533 Glycoprotein 2 (zymogen granule membrane) GP2 GP2_HUMAN Pancreatic secretory granule membrane major glycoprotein GP2 Pancreatic zymogen granule membrane associated protein GP2 Pancreatic zymogen granule membrane protein GP-2 ZAP75 |
| Molecular Weight(MW) | 59kDa; 59kDa(Calculated). |
| Cellular Localization | Cell membrane,Cytoplasmic vesicle,Endosome,Membrane,Secreted |
WB
Western blot analysis of extracts from Rat brain, using Glycoprotein 2 Antibody.
ICC/IF
Glycoprotein 2 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
