WB
Western blot analysis of PUMA in Jurkat, THP-1, C6, 3T3, Hela lysates using PUMA antibody.IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PUMA antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.ICC/IF
Immunocytochemistry analysis of PUMA (green) in 293T using PUMA antibody,and DAPI(blue).| Product Name | Rabbit Monoclonal Antibody to PUMA |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of human PUMA |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:100 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BBC3 |
|---|---|
| Gene Synonyms | JFY1 PUMA JFY-1 |
| Gene Full Name | BCL2 binding component 3 |
| Gene Summary | This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] |
| Alternative Names | BBC3 PUMA Bcl-2-binding component 3 JFY-1 p53 up-regulated modulator of apoptosis |
| Molecular Weight(MW) | 21kDa |
| Cellular Localization | Mitochondrion. |
WB
Western blot analysis of PUMA in Jurkat, THP-1, C6, 3T3, Hela lysates using PUMA antibody.
IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PUMA antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
ICC/IF
Immunocytochemistry analysis of PUMA (green) in 293T using PUMA antibody,and DAPI(blue).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:100 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
