WB
Western blot analysis of extracts from various samples, using GOLPH3 antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: Rat brain; Lane 3: Mouse brain.IHC
GOLPH3 Antibody at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
GOLPH3 Antibody staining 3T3 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to GOLPH3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human GOLPH3(Accession Q9H4A6), corresponding to amino acid residues L242-V292. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GOLPH3 |
|---|---|
| Gene Synonyms | GOPP1 GPP34 MIDAS Vps74 |
| Gene Full Name | golgi phosphoprotein 3 |
| Gene Summary | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | Coat protein Coat protein GPP34 FLJ90675 Golgi associated protein Golgi peripheral membrane protein 1 34 kDa Golgi phosphoprotein 3 (coat protein) Golgi phosphoprotein 3 Golgi protein GOLP3_HUMAN Golph3 GOPP1 GPP34 MIDAS Mitochondrial DNA absence factor |
| Molecular Weight(MW) | 34kDa |
| Cellular Localization | Cell membrane,Endosome,Golgi apparatus,Membrane,Mitochondrion. |
WB
Western blot analysis of extracts from various samples, using GOLPH3 antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: Rat brain; Lane 3: Mouse brain.
IHC
GOLPH3 Antibody at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
GOLPH3 Antibody staining 3T3 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
