WB
Western blot analysis of extracts from various samples, using NT Antibody. Lane 1: Mouse brain, blocked with antigen-specific peptides. Lane 2: Mouse brain. Lane 3: Hepg2 cells(heat-shock treatment). Lane 4: Hela cells(heat-shock treatment).ICC/IF
Immunocytochemical evaluation of CHD3 (green), RDH10 (green), NTM (green), and αSMA (red) in the control (Control) MSCs and after incubation in profibrotic (F) or adipogenic conditions (Ad). Fluorescent microscopy, blue staining—nuclei stained with DAPI, scale bar is 50 µm (A). The percentage of CHD3-positive cells, mean ± SE, p value is indicated (B). The percentage of cells with neurotrimin clusters, mean ± SE, p value is indicated (C).| Product Name | Rabbit polyclonal antibody to NT |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human NTM(Accession Q9P121), corresponding to amino acid residues P234-N284. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NTM |
|---|---|
| Gene Synonyms | HNT NTRI CEPU-1 IGLON2 |
| Gene Full Name | neurotrimin |
| Gene Summary | This gene encodes a member of the IgLON (LAMP, OBCAM, Ntm) family of immunoglobulin (Ig) domain-containing glycosylphosphatidylinositol (GPI)-anchored cell adhesion molecules. The encoded protein may promote neurite outgrowth and adhesion via a homophilic mechanism. This gene is closely linked to a related family member, opioid binding protein/cell adhesion molecule-like (OPCML), on chromosome 11. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| Alternative Names | hNT IgLON family member 2 IGLON2 Neurotrimin NT NTM NTRI_HUMAN |
| Molecular Weight(MW) | 42kDa; 38kDa(Calculated). |
| Cellular Localization | Cell membrane,Membrane. |
WB
Western blot analysis of extracts from various samples, using NT Antibody. Lane 1: Mouse brain, blocked with antigen-specific peptides. Lane 2: Mouse brain. Lane 3: Hepg2 cells(heat-shock treatment). Lane 4: Hela cells(heat-shock treatment).
ICC/IF
Immunocytochemical evaluation of CHD3 (green), RDH10 (green), NTM (green), and αSMA (red) in the control (Control) MSCs and after incubation in profibrotic (F) or adipogenic conditions (Ad). Fluorescent microscopy, blue staining—nuclei stained with DAPI, scale bar is 50 µm (A). The percentage of CHD3-positive cells, mean ± SE, p value is indicated (B). The percentage of cells with neurotrimin clusters, mean ± SE, p value is indicated (C).| Application Notes | WB:1:500-1:1000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
